Centrifugation Techniques and the Isolation of Cauliflower Mitochondria (1331 words, 8 pages)
The purpose of this lab was to practice and observe the isolation, separation, and purification of plant mitochondria in conjunction with centrifugation techniques and marker enzymes.Experimental Data ExplanationTable 1 Cuvettes For Assay of Succinate DehydrogenaseReagentCuvette No.123456Premix500L500L500L500L500L500LDCPIP----50L50L50L50L50L0.2 M Na-succinate50L50L50L100L50L50L0.2 M Na-malonate--------100L------------Mitochondrial Fraction----20L20L20L--------Whole Extract--------------------20LCytosol Fraction----------------20L----Water (to 1mL)450L380 L280 L330 L380 L380 LTable ...
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